FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_548-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_548-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7922703
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG195600.24688543796227122TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT47150.031.25030950
TGCCGTC49950.030.54955549
GTCACGG55300.029.61947430
AGTCACG58100.028.49322929
CATCTCG54300.027.3287640
CTGCTTG56300.027.16607758
CACGGTA61800.026.67405732
TATGCCG57700.026.50693747
GTATGCC58700.026.47273346
TCACGGT62650.026.3121631
CCGTCTT56650.026.13330751
CGGTAGC64750.025.40457734
CGTATGC61050.025.39638945
ATGCCGT60000.025.31584248
GCACACG67850.025.17235812
ACACGTC69000.024.7528214
ACGTCTG68700.024.60618416
GCATCTC60650.024.40975839
CTCGTAT61850.024.21909743
CACGTCT70200.024.18012415
Produced by FastQC (version 0.11.5)