FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_1664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_1664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11033714
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG357080.323626296639554TruSeq Adapter, Index 5 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG161550.020.94922358
GCTTGAA171350.020.91530860
TGCTTGA170650.019.97565859
CGTGTGC24800.018.7826771
AAAAAGG192400.017.88120870
GCCGTCT182750.017.86786750
CCGTCTT190200.017.59121551
TGCCGTC197000.017.57027249
ATGCCGT204950.017.00825748
TATGCCG211050.016.84832647
TCTGCTT213100.016.30850857
GTATGCC227050.015.92308646
CGTCTTC210050.015.89549652
GATCTCG181550.015.63410540
ACGACGC25100.015.61682416
CTCGTAT185100.015.58006343
CTTCTGC216650.015.50818455
CGACGCT25850.015.29911417
CTTGAAA241650.015.20725861
AGGGGGG13900.015.1179881
Produced by FastQC (version 0.11.5)