FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_1664-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_1664-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11833042
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG323190.27312503412055833TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG136950.018.14458758
GCTTGAA147400.017.59427860
TGCTTGA146450.017.15875859
GCCGTCT163450.015.5454250
TGCCGTC166650.015.22591749
CCGTCTT167250.015.17129551
ATGCCGT176300.014.45206348
TATGCCG177700.014.41698447
CGTCTTC186650.013.74443152
GTATGCC189000.013.4439146
CGTGTGC18100.013.3501341
TCTGCTT193000.013.16527957
CTTCTGC199750.012.84304355
CGTATGC202100.012.62443645
CTCGTAT201450.012.613049543
TATCTCG200500.012.49825640
AAAAAGG184000.012.23049270
CTTGAAA222800.012.20552961
TCGTATG209650.012.13641344
ATCTCGT211550.012.01086741
Produced by FastQC (version 0.11.5)