FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_1644.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_1644.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11667868
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG956000.81934420238556TruSeq Adapter, Index 2 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163680.14028269774735197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA254400.029.09653960
CGTGTGC41550.029.079351
AAAAAGG255250.029.04077770
CTGCTTG254700.027.57823658
TGCTTGA260550.027.14708959
CTTGAAA310000.024.40855461
TCTGCTT303600.023.7473657
CTTCTGC303800.023.59348555
CCGTCTT312700.023.36966751
CGTCTTC312750.023.04140352
GTGTGCT52950.023.0113412
GCCGTCT313950.022.67463750
TGCCGTC340450.022.29749749
TTCTGCT305400.022.18636956
GTCTTCT317300.022.0602253
ATGCCGT356400.021.40763548
TCTTCTG339050.020.8102254
TATGCCG376100.020.68636547
GTATGCC402700.019.59813946
TGAAAAA444500.019.47941863
Produced by FastQC (version 0.11.5)