FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_1644-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_1644-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10958032
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG1069160.9756861451034273TruSeq Adapter, Index 3 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185760.1695194903610429No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA264450.033.21857560
AAAAAGG251200.031.13957470
CTGCTTG278150.030.11039558
TGCTTGA282050.030.10339759
CGTGTGC38600.029.848131
CTTGAAA320450.027.70837461
TCTGCTT322400.026.36849257
CTTCTGC324350.026.20996555
CCGTCTT332750.025.76907751
CGTCTTC329500.025.72595652
CGACGCT33300.025.43442717
ACACGAC34650.025.15051814
TTCTGCT329300.025.08263256
GCCGTCT340600.024.8771750
GTCTTCT335500.024.6295453
CCCTACA35200.024.26051110
TGCCGTC366950.023.89195349
GTGTGCT49750.023.5761572
CTACACG36950.023.39555412
TCTTCTG358300.023.2380954
Produced by FastQC (version 0.11.5)