FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_1640.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_1640.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8993223
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG627360.6975919534075826TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG161250.030.1693847
GCCGTCT158750.030.00514250
TGCCGTC162350.029.83562749
ATGCCGT165500.029.24660748
CTGCTTG165550.028.85739958
GTATGCC169750.028.80301346
CCGTCTT167850.028.48266851
CATCTCG165550.028.30757740
CTCGTAT173050.027.68746443
GCTTGAA175950.027.64883660
CACGGCT178700.027.38018232
CGTATGC179250.027.31554245
TGCTTGA175150.027.31553559
GTCACGG179550.027.19208730
TCACGGC179900.027.10027531
CGGCTAC180150.027.06251534
ACGGCTA181750.026.93996433
AGATCGG192100.026.9072151
AGTCACG182350.026.88970829
ATCTCGT176400.026.54659841
Produced by FastQC (version 0.11.5)