FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_1567.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_1567.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10104203
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG3014242.9831546337697294TruSeq Adapter, Index 8 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG469410.4645690511166492No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATG420610.41627231756923333TruSeq Adapter, Index 8 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200030.19796712318626222No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATG192940.1909502412016069TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATT145530.1440291728105621TruSeq Adapter, Index 8 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC126690.12538346666233843TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTG122810.12154348047045373TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATG118480.11725813505528344TruSeq Adapter, Index 8 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG560950.052.3091670
AGGGGGG35500.041.7275351
AAAAAAG748550.040.63025769
CGTGTGC131650.035.0062641
GCTTGAA722800.034.11733660
ACGTGTG46400.034.1138841
CTACACG58400.033.8001812
ACGACGC58450.033.71122416
CTTGAAA764150.032.97649861
GTGTGCT140600.032.89612
ACACGAC61300.032.77192714
CGACGCT59500.032.7633917
CCTACAC60300.032.67712411
TCCCTAC60250.032.5299769
TGCTTGA718700.032.0426859
TGAAAAA1017700.031.7556363
CCCTACA62300.031.628110
CTGCTTG717550.031.35265558
TTGAAAA844150.030.90025962
GAAAAAA1086850.030.47262264
Produced by FastQC (version 0.11.5)