FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_1558.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_1558.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9037236
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG483870.5354181300565792TruSeq Adapter, Index 7 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC131000.029.70868349
ATGCCGT133950.028.9760248
TATGCCG138400.028.60068347
GCCGTCT125600.028.45040350
CTGCTTG128100.028.44143558
GTATGCC142500.028.17075546
CATCTCG137700.027.55150240
TGCTTGA135900.027.45286459
CCGTCTT135000.026.9360651
AGATCGG193300.026.7065891
GCTTGAA149700.026.06771560
CTCGTAT138900.026.00323543
GATCGGA199300.025.8781242
ATCTCGT143500.025.75502441
GCACACG201950.025.56219112
CGTATGC156350.025.33952345
CACACGT204600.025.2995313
ACGTCTG204900.025.2624916
ACACGTC204950.025.22217414
CACGTCT207000.025.05692515
Produced by FastQC (version 0.11.5)