FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_1557.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_1557.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8933159
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG823210.921521714770777TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA224200.029.17615360
GCCGTCT222550.028.96785550
TGCCGTC223400.028.8889749
TGCTTGA224350.028.7822459
CTGCTTG224250.028.6702258
ATGCCGT227000.028.49249348
TATGCCG228300.028.42222847
GTATGCC232450.027.8997446
CCGTCTT232700.027.79456751
CTCGTAT234600.027.03239643
GATCTCG227550.027.02397540
ATCTCGT233600.026.39899441
CGTCTTC250050.025.90799952
AGATCTC238450.025.89140339
CGTATGC251300.025.86269245
CGATCAG256000.025.51091234
AGTCACG259700.025.40393429
GTCACGA259700.025.36336330
TCGTATG255200.025.33031344
TCTCGTA248250.025.26405742
Produced by FastQC (version 0.11.5)