FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_1439.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_1439.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10257097
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG2884152.8118579750196373TruSeq Adapter, Index 13 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193110.1882696439353162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA485900.048.03591560
AAAAAGG416150.046.07065670
TGCTTGA507700.045.4287259
CTGCTTG522550.044.0841358
CTTGAAA538350.043.71346361
TCTGCTT584050.039.60390557
TGAAAAA608400.039.41095763
TTGAAAA604500.039.19046862
CGTGTGC41600.038.304131
CTTCTGC609150.038.006555
TTCTGCT617100.037.2900156
CCGTCTT630250.036.93400251
CGTCTTC633200.036.72323652
GAAAAAA670900.036.26116664
GTCTTCT636100.036.22569353
AAAAAAG546900.036.01624769
TCTTCTG647400.035.690754
GCCGTCT655300.035.49008650
AGGGGGG20400.034.849251
TGCCGTC676800.034.6574349
Produced by FastQC (version 0.11.5)