FastQCFastQC Report
Tue 6 Aug 2019
HN22FAFXY_n01_1215a-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22FAFXY_n01_1215a-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11317546
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG3223412.848152770927549TruSeq Adapter, Index 4 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG363570.3212445524851412No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138240.12214662083105295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG481000.045.4979970
AGGGGGG29250.037.8350331
CGTGTGC60500.036.2369651
AAAAAAG640450.035.06129569
GCTTGAA803150.032.3032560
CTTGAAA825550.031.64295861
ACGACGC49300.031.37792216
CTACACG50000.030.86863312
CGACGCT49700.030.77328917
CCTACAC50850.030.21498511
TGCTTGA873300.029.41585459
ACACGAC54600.029.03717814
TTGAAAA906050.028.92427462
TGAAAAA920900.028.91771363
CTGCTTG897600.028.5649158
CCCTACA54750.028.51015510
GTGTGCT77000.028.4659862
TCCCTAC54400.028.3722829
GACGCTC53050.028.23626318
CACGACG55550.028.22557415
Produced by FastQC (version 0.11.5)