FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n02_spbs_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n02_spbs_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944651
Sequences flagged as poor quality0
Sequence length150
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAT450.00990092148.0082933
GTAGACT1157.479519E-637.5755774
CTCTATA2053.1468517E-1035.1253052
TACTGTG1051.9276074E-434.2969327
TAGGATA1102.5339224E-432.7362944
TAAGACT900.00374908632.0088234
GTATAAG950.00488702930.3210281
TTAAAGT1000.006276307628.8079414
TAAGAGA1504.6048044E-528.8079414
TATAGGG2003.4574805E-728.8027522
ATGTATG3103.092282E-1127.8757821
TTAGAGT1050.0079642927.4361324
TAATGTA1050.0079642927.4361324
GTTATAA1358.4104785E-426.6712721
GTGTAAA2456.880691E-826.4535471
AGGGTAG1100.00999219326.1890355
TAAACTC1400.00103932225.7213765
TATAGGA1400.001040414625.7167452
GTATTTA2259.667383E-725.6044221
CTATACT2001.12252055E-525.206954
Produced by FastQC (version 0.11.2)