FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n02_spbs_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n02_spbs_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886700
Sequences flagged as poor quality0
Sequence length150
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTT1753.975143E-628.8101165
TATAGGA1504.6140292E-528.7994212
CGTAAGA1050.00797573227.428022
CTAATCT1607.143696E-527.0094834
TAGACTA1100.00998844226.1910155
ATAGTAC1100.00999877526.1854573
ACTAAAC1400.001038692125.7248
TTAGACT1701.07886604E-425.420694
CTAGACG1450.001274733324.8363084
CTAGGAC1751.3154923E-424.6891463
GTATAAG2051.36070885E-524.5887831
GTATAGG3052.1891537E-823.609841
ATAAGAC1851.9176374E-423.3545973
TAAGACA3554.456524E-922.3176964
CTATACT1952.7348087E-422.1616274
GTATAGA3905.475158E-1022.1569251
TATATGA1952.741685E-422.1534022
TATAGTG1650.002702839421.8258465
TAGGATT1650.002702839421.8258464
CTATAGT1700.003213936521.1839084
Produced by FastQC (version 0.11.2)