FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n02_slp_neg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n02_slp_neg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53832
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7771.4433794025858224No Hit
ATGCTGTCTCTTATACACATCTGACGCTGCCGACGACGGAGAGAGTGTAG550.10216971318175064No Hit
AGATGCTGTCTCTTATACACATCTGACGCTGCCGACGACGGAGAGAGTGT550.10216971318175064No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG100.0072202063144.000024
GTATTAA100.0072202063144.000021
TAGCGAG100.0072202063144.000024
ATTAAGG100.0072202063144.000023
AGGCGTA100.0072202063144.000024
CCTAAGT100.0072202063144.000022
TTATCCG100.0072202063144.000022
GTAATGG100.0072202063144.000023
CGTAGTG100.0072202063144.000027
TACTACC100.0072202063144.000027
TCTCAGT100.0072202063144.000028
GATTAAG100.0072202063144.000022
CACTTTA100.0072202063144.000029
CTCAGTA100.0072202063144.000029
CGAGTTA203.9533342E-4108.000019
CAGGACT203.9533342E-4108.000014
ATCTCTC259.5984765E-486.3999947
CTTCCCC259.5984765E-486.3999949
AGGACTT259.5984765E-486.3999945
TAAGTCC259.5984765E-486.3999946
Produced by FastQC (version 0.11.2)