FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n02_s8_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n02_s8_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83441602
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA1715850.016.6703171
GTATTAT1018100.016.6152361
GTATTAG733700.016.538471
GTATAGG391850.015.547641
GTATTAA887650.015.4954981
GTATAGA632850.015.3961091
TATACAG866350.014.963665
GTGTATA1320550.014.593021
GTATAAA1110250.014.5162091
TTATACT1041250.014.5111964
GTATAAG661050.014.4342921
TTAGCGA331450.014.0367724
CTTAGCG329400.013.96894553
GTATAAT1011800.013.9429181
TCTATAC606950.013.8097693
TTAGGGT747650.013.7749624
GGGTTAG537550.013.691341
TATACTG948050.013.5829785
TAGCGAA345100.013.5443595
ATAATAC1033550.013.4535563
Produced by FastQC (version 0.11.2)