FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n02_s7_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n02_s7_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85554221
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA1720550.015.7250271
GTATTAG762400.015.4909651
GTATTAT1069300.014.6277581
GTATTAA963600.014.2518571
GTATAGG429100.014.1309411
GTATAGA660350.014.10073951
TATACTG919700.013.9311575
TATACAG910200.013.8787435
GTGTATA1323050.013.7436691
CTTAGCG488100.013.6170333
TTAGCGA496800.013.5979154
GTATAAG719350.013.4147351
ATAGGGC867050.013.4044493
TATAGGG987250.013.3974412
GTATAAA1195150.013.3706461
TAGCGAA511650.013.076765
TAGGGCC802950.013.0235444
TCTATAC629950.012.8941473
CTATAGG1035150.012.828481
TTATACT1083100.012.7934484
Produced by FastQC (version 0.11.2)