FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n02_s4_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n02_s4_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82176804
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGG1596500.018.0756192
TAGGGCC1431550.017.8651354
CTATAGG1638450.017.554571
ATAGGGC1496450.017.5542093
TTAGCGA1139600.016.9562724
CTTAGCG1153100.016.6362673
TAGCGAA1170400.016.467195
GGGCCAA1544850.016.359646
CGAAAGG1145800.016.2308528
GCCCTAT1180800.015.9542631
AGGGCCA1629250.015.9406385
GCGAAAG1178400.015.9405457
GTATTAG669950.015.8442051
GTATATA1572400.015.841741
CCCTATA1295650.015.6931962
AGCGAAA1214250.015.5703336
CTATAGT1495300.015.3550824
CCTATAG1351400.015.1968743
GTATAGG388700.015.1178821
GTCTTAG1749650.015.1094461
Produced by FastQC (version 0.11.2)