FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n02_s3_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n02_s3_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82207754
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGA649800.016.3257714
GTATATA1471600.016.202371
CTTAGCG655250.016.0337543
TAGCGAA664350.016.0226065
GTATTAT941250.015.8906131
GTATTAA820550.015.7882971
GTATTAG646550.015.6266081
CGAAAGG665500.015.4764158
GCGAAAG675100.015.4054117
GTCTTAG1122100.014.9660151
TATAGGG999250.014.9082442
GTATAAG564550.014.8222071
AGCGAAA711150.014.7253466
TATACAG771350.014.51892955
TAGGGCC858350.014.4903414
ATAGGGC914900.014.4584753
GTATAGG366200.014.4340061
CTATAGG1046600.014.4218081
GTATAAA1038400.014.251361
GTATAGA555600.014.0240741
Produced by FastQC (version 0.11.2)