FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n02_s1_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n02_s1_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94831785
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG746200.016.1644061
GTATATA1880100.016.0637781
TTAGCGA1246350.015.8908354
GTATAGG438050.015.6664071
TATAGGG1739800.015.572772
CTTAGCG1262650.015.5234793
TAGCGAA1273350.015.4805145
CGAAAGG1265600.015.4396648
TAGGGCC1535150.015.3831274
ATAGGGC1609550.015.3049723
GCGAAAG1295350.015.2905937
CTATAGG1776150.015.1916261
GTCTTAG1827850.015.0061951
AGCGAAA1325850.014.9167886
GTATAGA647800.014.8735751
GTATTAT1115600.014.8657081
GTATTAA968700.014.8527321
GCCCTAT1280700.014.4224871
CCCTATA1416900.014.2891742
GGGCCAA1666050.014.287386
Produced by FastQC (version 0.11.2)