FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n01_spbs_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n01_spbs_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944651
Sequences flagged as poor quality0
Sequence length150
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATC450.00990800247.9996074
GTATAAG1355.236252E-737.347431
GTTAACA850.00283701833.8829422
TAATAGT1501.1977609E-633.5997244
TATACAG2404.7293724E-1132.999735
GTCTAGT900.003747216432.0120851
CCCTAAG1000.006273184428.8108771
TTATAAG1504.6129197E-528.8005032
TTAGCCA1255.368954E-428.7997654
CTAACCC1000.006285017428.7997654
TACTAAT1000.006285806728.7990237
ACATTAC1306.738042E-427.7027661
GTCTAGA1306.738042E-427.7027661
TGCTATA1050.0079743327.4290522
GTCTATA2654.8930815E-927.1800731
GCCCTAT2705.973561E-926.6767371
ATCATAG1358.4004237E-426.6767371
TAGGTTG1358.419371E-426.6664496
TAGGGGT1358.419371E-426.6664494
ATATACG2207.932322E-726.1917061
Produced by FastQC (version 0.11.2)