FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n01_spbs_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n01_spbs_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886700
Sequences flagged as poor quality0
Sequence length150
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA1501.1942684E-633.6121861
GTATTTA2151.9317667E-830.1504651
TAGGGTA1204.2288718E-429.9981547
GTCTAGA1453.65186E-529.8039111
CTAAAGT1453.6617832E-529.7920634
GTCTAAT1255.357255E-428.8104461
CCTAGAC1255.3689023E-428.7997573
ATTAAGG2003.4605364E-728.7997553
TTAGCAC1555.7689722E-527.8707333
GTACTAA1306.7385565E-427.7023531
TTAAGAC1306.753192E-427.6920723
GTATTAC3700.027.2531241
TAAGGTT1607.162496E-526.9990564
TAAGGGA1607.162496E-526.9990564
CCTAAAG1358.4192905E-426.666443
TAGGGAC1358.420594E-426.6657334
TAGTCTA1358.420594E-426.6657335
GTATAGA3659.094947E-1225.6531351
CCCTACA2301.1726988E-625.0432662
ACAATAC2601.2253076E-724.9228653
Produced by FastQC (version 0.11.2)