FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n01_slp_neg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n01_slp_neg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53832
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT3310.6148759102392629No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC1500.27864467231386536No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAT100.0072202063144.000023
CTTCATA100.0072202063144.000024
TCTTACG100.0072202063144.000023
CAACATA100.0072202063144.000029
TTTCAAT100.0072202063144.000021
TACATAG100.0072202063144.000025
GCTCTAA100.0072202063144.000027
CCTTTAC100.0072202063144.000021
TGCCTCT100.0072202063144.000026
ATAGTTG100.0072202063144.000028
TAGGACA100.0072202063144.000024
CGACTAG100.0072202063144.000029
TATGTGT100.0072202063144.000024
GATGAGA151.257797E-4144.06
ATATACG203.9533342E-4108.000011
GTCTATG259.5984765E-486.3999946
AATATTC259.5984765E-486.3999945
AACCTCG259.5984765E-486.3999947
ACACTTT259.5984765E-486.3999948
CACACTT259.5984765E-486.3999947
Produced by FastQC (version 0.11.2)