FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n01_s8_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n01_s8_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83441602
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA1683800.017.1136321
GTATTAT995950.015.8947731
TATACAG873400.015.8353865
GTATTAG727150.015.748431
GTGTATA1222050.015.3644221
GTATAAA1092650.015.14063551
GTATTAA884500.015.088351
GTATAAG638050.014.9337651
GTATAGA616150.014.7398411
GTATAAT945950.014.6183121
TAATACT998200.014.2955074
GTATAGG400150.014.2909761
ATAATAC1025950.014.1544893
TATTATA1498100.014.0764232
TTATACT1052300.013.9710694
TCTATAC598500.013.8941623
TATACTG959800.013.8323095
TATAATA1423350.013.7736692
ATTATAC1034350.013.5174943
TATAGGG750400.013.5090112
Produced by FastQC (version 0.11.2)