FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n01_s7_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n01_s7_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85554221
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA1690600.015.9842731
GTATTAG754650.014.6880421
GTATAGG421200.014.5003461
GTATTAT1058950.014.2420381
GTGTATA1277150.013.9686681
TATACTG904050.013.7934535
GTATAGA647650.013.4559971
TATACAG915450.013.4486615
GTATAAG703500.013.3705711
GTATTAA932950.013.3554531
GTATAAA1174700.013.3291811
GTATAAT986950.013.2304171
TATAGGG981450.013.219172
ATAATAC1084500.012.8792253
ATAGGGC870500.012.6212783
TAGGGCC805500.012.5313984
TATATAC1737200.012.3256183
CTATAGG1042950.012.1747411
CTTAGCG525550.012.0966253
TAATACT1041300.012.0864
Produced by FastQC (version 0.11.2)