FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n01_s4_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n01_s4_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82176804
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGG1621600.018.4166972
ATAGGGC1515450.018.0770763
TAGGGCC1449300.018.0080094
CTATAGG1665450.017.4753951
GGGCCAA1573650.016.6674696
AGGGCCA1661650.016.2526445
GTATAGG381750.015.9799041
TTAGCGA1182600.015.8715424
GCCCTAT1212750.015.6962411
CTTAGCG1186350.015.6574873
TAGCGAA1201050.015.6037715
GTATTAG665750.015.4593361
CCCTATA1341700.015.4490942
GTATATA1532300.015.3417931
CGAAAGG1184800.015.100478
CTATAGT1525800.014.9675794
GCGAAAG1213950.014.963217
AGCGAAA1238050.014.8525616
CCTATAG1406500.014.8242933
GTATAGA558750.014.6172361
Produced by FastQC (version 0.11.2)