FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n01_s3_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n01_s3_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82207754
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA1445900.016.0447351
TTAGCGA663100.015.2552434
GTATTAG646700.015.1800461
TATAGGG1006350.015.0027622
TAGCGAA672700.014.8876985
CTTAGCG668150.014.8059383
GTATTAT917750.014.75412751
ATAGGGC926850.014.6039913
TAGGGCC868950.014.4667614
GTATTAA816650.014.4463461
GTATAAA1023950.014.3633941
CTATAGG1053450.014.2688381
GCGAAAG691550.014.2316467
TATACAG767900.014.1483695
CGAAAGG683150.014.1115258
GTATAGG350650.013.98792651
GTATAAT859050.013.8506991
AGCGAAA719300.013.8030646
GTATAAG563350.013.7694791
GTGTATA1036000.013.7583431
Produced by FastQC (version 0.11.2)