FastQCFastQC Report
Thu 16 Nov 2017
HN22CBGX3_n01_s1_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN22CBGX3_n01_s1_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94831785
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA1851950.016.065481
TATAGGG1760050.015.9536172
TAGGGCC1570850.015.6980084
CTATAGG1799250.015.54731751
ATAGGGC1652100.015.4881563
GTATTAG738500.015.233451
TTAGCGA1314900.014.641564
GGGCCAA1695750.014.6352216
GTATAGA620200.014.6320951
GTATAGG426450.014.4061751
TAGCGAA1345350.014.3530135
CTTAGCG1332850.014.28772453
GCCCTAT1323400.014.2858451
CGAAAGG1327200.014.2614888
TATACAG929100.014.1732955
AGGGCCA1792550.014.0537495
GTATAAG647250.013.9983331
GCGAAAG1374250.013.9879957
GTATTAA976600.013.9236481
AGCGAAA1384150.013.8882636
Produced by FastQC (version 0.11.2)