FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77368612
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14424531.8643904326472858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT75500.020.4376264
TCATTAA854500.019.2297194-95
TATCATT1283950.018.402194-95
GTATCAT1528150.017.56004794-95
CGTATCA1805700.016.65277194-95
TAGACGG47700.016.1267645
GTCTATG121100.015.7653631
GTACCGT66650.015.7451866
AGTACCG86000.015.5152385
CCGTATC2122750.015.49391394-95
ATCATTA1407250.015.09828394-95
CACTGAG144000.015.0290117
GTATAGG140050.014.2086531
ACTGTCC72750.014.1561348
GCCGTAT2619750.014.10150594-95
TACCGTG74900.014.0669277
GTGTATG171050.013.6881861
TACACCG85850.013.6064675
CGCCGTA3109300.013.46507794-95
AAGACCG96300.013.3133735
Produced by FastQC (version 0.11.5)