FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_SAL60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_SAL60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77292994
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG96550.029.6165055
GTCTAGG240450.021.6200751
GACGGTC120750.021.2800967
TATACCG277950.020.6950785
TAGAGCG228600.019.6356455
CCTAGAC240300.018.9761033
GGACTGC187650.018.9086996
GTACCGT142200.018.5054536
GTCTATG296150.018.3881281
ATAGACG136500.018.0254754
AGTACCG198750.017.9004635
CTAGACA301100.017.7472614
GTATCAT1999350.017.7446194-95
CGTATCA2336200.017.72368494-95
CCGTATC2800600.017.52827894-95
ACTGAGC307650.017.3220888
CACTGAG343300.017.0451327
TATCATT1784200.016.69477394-95
ATCATTA1482500.016.69417494-95
ACTGTCC145350.016.665548
Produced by FastQC (version 0.11.5)