FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_SAL5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_SAL5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108233362
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG117000.033.0059935
TATACCG349250.023.5286945
GTCTAGG325350.023.383661
GTACCGT179300.023.0741776
GACGGTC159350.021.9971287
AGTACCG263700.021.020785
TAGAGCG295500.020.9447425
CTAGACA356850.020.871254
GGACTGC237900.020.8047076
GTCTATG380350.020.601991
ATAGACG167700.020.4216254
CCTAGAC324900.020.40913
CACTGAG437900.020.033357
ACTGAGC387150.019.9359428
TACACCG235850.019.797295
GCCCTAG194150.019.776341
ACTGTCC179250.019.6345838
ATCATTA2278500.019.40998894-95
GTATCAT3295850.019.36687394-95
TATCATT2820950.019.2248294-95
Produced by FastQC (version 0.11.5)