FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_SAL5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_SAL5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92994044
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA2804500.021.82058194-95
ATCATTA3614600.020.75844294-95
CATTAAA2060950.020.11265294-95
TAGACGG108500.019.0873015
TATCATT4491550.018.86722694-95
GTACCGT159300.018.486896
TATACCG253700.018.2171365
GTATCAT5327550.017.79865694-95
AGTACCG206300.016.9229415
GTCTAGG257900.016.5830571
CGTATCA6407400.016.47490594-95
CCGTATC7652800.015.45177994-95
TACCGTG195450.015.0910237
GTCTATG292400.014.951471
ACTGTCC172850.014.9208698
TACACCG196700.014.7545045
CCTAGAC265050.014.2471193
GCCGTAT9311050.014.14370494-95
TAGAGCG225600.014.1276915
GTATAAG376900.014.1083871
Produced by FastQC (version 0.11.5)