FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_SAL180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_SAL180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94467271
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG99550.026.4812365
GTCTAGG262150.022.4410321
TATACCG272050.021.527865
ATCATTA2246550.019.98501894-95
GTACCGT145150.019.9290586
CCTAGAC270800.019.3294493
GTATCAT3291650.019.28890894-95
TACACCG185700.019.1838555
AGTACCG202900.019.1261045
TATCATT2799200.019.0744694-95
ACTGTCC144150.018.9789858
TACCGTG178000.018.9720217
TCATTAA1803650.018.76648594-95
CACTGAG328250.018.694847
GTCTATG297750.018.65671
GGACTGC188750.018.5215536
CTAGACA271750.018.5102774
CGTATCA4024750.018.32603594-95
ACTGAGC301700.018.3092088
TAGAGCG237250.018.2789425
Produced by FastQC (version 0.11.5)