FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_SAL15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_SAL15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90893817
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG101400.029.8391235
GTCTAGG264500.023.8225041
GTACCGT142500.021.7662126
TATACCG273050.021.6576735
GACGGTC127600.020.2865837
CCTAGAC265600.020.011823
ACTGTCC144450.019.9259438
ATCATTA2038650.019.8537994-95
AGTACCG209850.019.420615
GTATCAT2995750.019.38651894-95
TACCGTG187950.019.2311577
TATCATT2559450.019.22521694-95
CTAGACA275750.019.1201844
GGACTGC194550.019.1167956
GTCTATG306600.018.8620111
GCCCTAG157900.018.8391691
ACTGAGC299950.018.4476418
TACACCG187800.018.4128235
AAGACCG217400.018.2873385
GTGTATG357300.018.1937661
Produced by FastQC (version 0.11.5)