FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_NON5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_NON5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60185424
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA1266600.017.17120294-95
TCATTAA1532700.017.06769494-95
CCGTATC3588750.016.565594-95
TATACCG173550.016.339285
CGTATCA2922450.016.2844394-95
GCCGTAT4534300.015.96112294-95
TAGACGG85300.015.7587075
GTATCAT2457700.015.03432494-95
CGCCGTA5450050.014.89518194-95
ATCATTA1801650.014.84545494-95
TAGAGCG170950.014.4483225
GTCTAGG156900.013.9303391
GTATAGG268900.013.8886021
TATCATT2122300.013.73954794-95
CCTAGAC153050.012.8484333
CTATACT297750.012.7301734
CCTATAC281400.012.710253
GTACCGT140750.012.587626
ACTGTCC142850.012.4687128
AGTACCG170000.012.26588255
Produced by FastQC (version 0.11.5)