FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_NON5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_NON5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65439295
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA2301150.020.67291594-95
CATTAAA1782500.020.6413194-95
ATCATTA2875500.018.77967594-95
TATCATT3526550.017.21734294-95
GTATCAT4230150.016.85945594-95
CGTATCA5086450.016.3895694-95
TATACCG159050.016.0072635
CCGTATC6146200.015.59821494-95
GCCGTAT7439700.014.63253994-95
GTATAGG269850.014.5804571
ATTAAAA1650000.014.45950194-95
CTATACT293050.014.0690244
GTCTAGG148150.013.8883121
TAGACGG84650.013.6914315
CGCCGTA8756050.013.3528494-95
GTATAAG221400.013.2639871
CCTATAC284600.013.2516993
AGTACCG156900.013.2295325
GTACCGT131750.013.1952146
ACTGTCC128350.012.1749568
Produced by FastQC (version 0.11.5)