FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_NON180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_NON180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98136397
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG114600.027.3143185
GTCTAGG288350.023.2028271
TATACCG287050.021.0484035
GTACCGT179600.019.253726
ACTGAGC364000.018.8030688
AGTACCG244550.018.8017045
CACTGAG381350.018.7571537
ATCATTA2250850.018.68251294-95
GTCTATG328200.018.3440951
CCTAGAC287050.018.235333
GTATCAT3215500.018.2283594-95
TAGAGCG261850.018.1944085
TATCATT2763600.018.09358894-95
TCATTAA1802150.017.84224594-95
GGACTGC228250.017.7303946
ATAGACG148100.017.704094
ACTGTCC170450.017.6110888
CGTATCA3870250.017.60238394-95
CTAGACA330350.017.4699444
GTGTATG388450.017.211441
Produced by FastQC (version 0.11.5)