FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_NON15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_NON15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179745943
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG182850.025.7954185
TCATTAA6741750.024.23941894-95
CATTAAA4730750.023.69381994-95
TATACCG494300.022.5533355
ATCATTA8959700.022.23339794-95
GTCTAGG453750.021.8870931
GTACCGT295650.020.7413316
TATCATT11213950.019.86803294-95
TAGAGCG434850.019.3230935
AGTACCG418050.019.1792895
ACTGTCC287000.018.8498138
GTCTATG530100.018.3940751
TACCGTG385750.018.2476377
GTATCAT13572450.017.89111194-95
CTAGACA513350.017.5156124
TACACCG340250.017.4362325
ACTGAGC608600.017.2864978
CACTGAG644650.017.1670747
GGACTGC368050.017.1645776
TAAGGTG559650.017.1019975