FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_EXW60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_EXW60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96501018
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG126650.024.0404915
GTCTAGG282400.022.3459721
TATACCG267850.021.9720295
GTCTATG322150.020.1639771
GTACCGT205250.020.0644136
ACTGTCC218700.019.9370028
ATCATTA1701650.019.39434894-95
TATCATT2113300.019.12981494-95
GTATCAT2497550.019.09668494-95
CTAGACA289200.019.0031494
AGTACCG273250.018.8955655
AACAGAC290550.018.5868137
TCATTAA1324650.018.33185894-95
CACTGAG541900.018.003077
CGTATCA3027950.017.94317494-95
CCTAGAC277800.017.902143
TAGAGCG307350.017.7109555
ACTGAGC500550.017.6209958
TATACTG348850.017.428575
GTATAAG364500.017.3518731
Produced by FastQC (version 0.11.5)