FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_EXW60-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_EXW60-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141712395
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG143100.027.3177785
TCATTAA4472450.024.79422894-95
CATTAAA3048450.023.45658594-95
ATCATTA6043600.023.44140894-95
TATACCG398200.022.675985
GTACCGT218500.022.5430416
GTCTAGG386800.021.0446411
TATCATT7662050.021.02033494-95
GTCTATG440250.019.5258451
TACCGTG273000.019.2247877
ACTGTCC221750.019.0629028
AGTACCG320200.018.9877645
CTAGACA405800.018.599324
GTATCAT9210250.018.56072894-95
TAGAGCG370550.018.1766825
CACTGAG557650.017.7925687
CCTAGAC385300.017.5794683
ACTGAGC497900.017.5047788
GTGTATG500750.017.2047161
GACTGTG142500.017.1654137