FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_EXW5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_EXW5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255588
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2570.10055245160179664No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG200.002072564871.247065
CAGGTCG459.612955E-442.2204865
TACTGTT901.1907105E-842.2204867
CTACGCA500.001613791837.9984369
CCGTAAC550.002576409134.5440339
GTCTAGA851.6125137E-533.5740131
TAAGGTG851.6277192E-533.528035
GTATCCG1051.8946394E-631.6653639
ACTGAGC753.072544E-431.6653618
GACTGCC753.072544E-431.6653617
GACTATG753.072544E-431.6653617
CCCTAGG600.00394605131.665362
CAGGGGA804.4850569E-429.6862779
ACTGCCT650.005836857529.2295678
CCTACGC650.005836857529.2295673
CTTAGAC650.005836857529.2295673
CCTTAGG1153.8094622E-628.9515041
TGTATAC1004.9396407E-528.4988253
CCTAGAC856.394986E-427.9400253
GTACCGT700.00838126227.1417396
Produced by FastQC (version 0.11.5)