FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_EXW5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_EXW5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71724449
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3113830.43413787675106436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC1364750.014.5076194-95
GCCGTAT1777200.014.40567194-95
TCATTAA751950.013.88861194-95
CGTATCA1115200.013.63710194-95
CATTAAA695900.013.55669194-95
CGCCGTA2274350.013.10309594-95
GTATTGG438350.012.1088881
TATACCG167950.012.1047385
GTATCAT1007700.011.62473694-95
TATACTG213450.011.1934375
ATCATTA838800.011.14520494-95
GTATAGG203950.010.8110131
ACTGAGC339600.010.5734798
GGACCGT249750.010.4794316
TATCATT977750.010.3937392-93
GTCTATG216300.010.3255561
GTCTTAG137400.010.2716881
AGTACCG213300.010.2214735
CCTAGAC211550.010.1488553
TTAGGAC219450.010.0648883
Produced by FastQC (version 0.11.5)