FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_EXW180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_EXW180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102431994
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1226830.11977019601902897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG137100.024.355825
GTCTAGG316250.021.3665621
TATACCG308700.020.3875085
GTCTATG368900.019.8628391
TAGAGCG347850.018.6937475
GTACCGT231650.018.6797476
CCTAGAC298200.018.3497333
ACTGTCC248000.018.1560448
GTATCAT1958650.017.73739494-95
CTAGACA340500.017.618594
CACTGAG628600.017.4089267
ACTGAGC584550.017.3964088
CGTATCA2305900.017.37664894-95
CCGTATC2752350.017.37385794-95
AGTACCG306850.017.3371145
AACAGAC330800.017.287217
GTGTATG388300.017.1204781
TATACTG393200.016.9121745
ATAGACG162050.016.8540294
GTATAAG418550.016.6778471
Produced by FastQC (version 0.11.5)