FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_EXW180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_EXW180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121459692
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2309740.19016514548711355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3709500.024.37708994-95
ATCATTA4969350.023.71639694-95
TAGACGG119350.023.1625085
CATTAAA2561850.022.57917694-95
GTCTAGG325100.021.8081111
GTACCGT203750.021.750496
TATACCG303250.021.5057585
TATCATT6285350.021.13484694-95
GTCTATG374950.019.9732631
AGTACCG282750.019.6211855
TAGAGCG333050.018.8541535
GTATCAT7596650.018.713294-95
ACTGTCC210500.018.0510778
CACTGAG543650.017.8402397
GTGTATG392350.017.8157941
TACCGTG248250.017.8124927
CTAGACA310650.017.7213634
CCTAGAC313900.017.5378823
ACTGAGC515800.017.413078
GTATAAG463800.016.8334451
Produced by FastQC (version 0.11.5)