FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_EXW15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_EXW15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83016180
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG861490.10377374627452143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG115500.021.426035
GTCTAGG275850.019.0002171
TATACCG253250.018.2494325
GTCTATG337450.018.0101411
CCGTATC1360250.017.13089694-95
CGTATCA1180950.016.97448294-95
GTATCAT1038000.016.75397794-95
AACAGAC296900.016.0935867
TAGAGCG345600.015.915525
CCTAGAC247600.015.8458443
ACTGTCC275600.015.8036068
CTAGACA304300.015.3595724
ATAGACG131850.015.0224884
GTGTATG344700.014.7777921
GACAGTG321500.014.7735237
GTATAAG320300.014.5090161
AGTACCG287400.014.3951895
GTACCGT236700.014.3480796
TATACTG304600.014.2060875
TACACCG217150.014.0430425
Produced by FastQC (version 0.11.5)