FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n02_EXW15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n02_EXW15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125247834
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2139970.1708588429561185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3753750.024.06948994-95
ATCATTA4997350.023.07829994-95
CATTAAA2632600.022.4252994-95
TATCATT6333100.020.7474894-95
TAGACGG136350.020.4490645
TATACCG297800.020.0174875
GTACCGT200950.019.6900886
GTATCAT7636700.018.65861194-95
GTCTAGG329200.018.129831
AGTACCG272350.017.492995
TACCGTG253000.017.2903847
GTATAAG469600.017.2224671
GTCTATG360050.017.1835171
ACTGAGC464450.017.0680438
CACTGAG499500.017.039947
ACTGTCC218650.016.7700448
CGTATCA9197750.016.2523594-95
CTAGACA314500.016.2510764
GTGTATG398100.016.1498891
TAGAGCG326300.016.0127545
Produced by FastQC (version 0.11.5)