FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77368612
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG44400.023.6010135
GTCTATG127800.021.8798181
GTACCGT60900.019.2311956
GTCTAGA117150.017.0202641
GTGTATG121450.016.8867281
AGTACCG82400.016.687535
CACTGAG143600.016.4087737
TATACTG143450.016.1963395
ACTGTCC65250.015.9124768
GTCTTAG85400.015.8989131
GTCGGTC159350.015.7092718
TACCGTG74800.015.5288277
GGTCGGT166000.015.1656357
GTATAGG137750.015.1642281
TAAGGTG111350.015.1167865
GCCCTAG78700.014.8998361
TACACCG82700.014.8496295
GTGTAGG83000.014.6998971
TCTAGAG164050.014.644352
GACAGTG78600.014.1748847
Produced by FastQC (version 0.11.5)