FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_SAL60-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_SAL60-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77292994
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG98600.028.7602335
GTCTAGG239800.022.6933061
TATACCG282000.021.2066295
GACGGTC127900.020.611147
TAGAGCG228950.020.0000655
GTACCGT149350.019.8460376
GTCTATG294000.019.3503251
CCTAGAC245600.019.2054043
GGACTGC193050.019.1919566
CTTCTGC1317200.018.50203794-95
AGTACCG196200.018.302815
ATAGACG137700.018.2481424
GTCTTCT1996950.017.70723394-95
CACTGAG348600.017.672267
TCTTCTG1705600.017.47763694-95
ACTGAGC318900.017.4563188
ACTGTCC147900.017.406478
CGTCTTC2413300.017.32139694-95
TTCTGCT1028900.017.3175194-95
TACACCG180950.017.2465325
Produced by FastQC (version 0.11.5)