FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_SAL5D-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_SAL5D-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108233362
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG118800.033.3056225
GTCTAGG327850.024.6878411
GTACCGT177300.024.2185366
TATACCG343200.023.154625
GACGGTC154650.023.1272837
AGTACCG256300.022.3690555
CCTAGAC331050.021.7952483
CTAGACA357650.021.4090794
ACTGTCC176900.021.2387148
CTTCTGC2116950.021.05226994-95
TAGAGCG300450.020.694575
GTCTATG384750.020.5179821
ATAGACG169000.020.5176354
CACTGAG437550.020.4518577
ACTGAGC386900.020.391568
TACCGTG225650.020.354987
GGACTGC249400.020.1501086
TACACCG231100.020.1425825
TTCTGCT1591900.019.99426794-95
TCTTCTG2828400.019.67671694-95
Produced by FastQC (version 0.11.5)