FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_SAL5D-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_SAL5D-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92994044
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT1907600.021.59089794-95
CTTCTGC2683900.020.80511794-95
TATACCG254950.019.1339915
TAGACGG105050.019.0812385
TCTGCTT1561850.018.979994-95
TCTTCTG3383200.018.7463394-95
GTCTTCT4035500.017.85967894-95
GTCTAGG259550.017.5788041
GTACCGT158300.016.6233486
CGTCTTC5282200.016.35940694-95
AGTACCG206450.016.2665215
GTCTATG289650.015.9325341
CCGTCTT6432300.015.65572594-95
GTATAAG382650.015.36411
TAGAGCG221600.015.1335
CTGCTTG1012800.015.10474794-95
TACCGTG192150.014.9799697
CCTAGAC259450.014.7929683
TCCGTCT2041800.014.78715294-95
GGACTGC217350.014.532886
Produced by FastQC (version 0.11.5)