FastQCFastQC Report
Mon 19 Oct 2020
HMYNCDRXX_n01_SAL180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYNCDRXX_n01_SAL180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50758566
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2775800.017.81713394-95
CCGTCTT2177750.017.00390494-95
TGCCGTC3444600.016.6304294-95
TATACCG150250.015.1430665
CGTCTTC1835750.014.52685594-95
TTCTGCT1009750.014.42586394-95
ATGCCGT3889100.014.13300494-95
TCTGCTT882550.014.05874594-95
GCTTGAA456750.013.86322594-95
CCTAGAC158150.013.7861143
TGCTTGA581150.013.7810494-95
CTGCTTG692350.013.35492494-95
GTCTAGG158250.013.27424051
TATGCCG4321650.013.2293392-93
TAGACGG76350.012.9403835
GTCTTCT1503250.012.68091794-95
GTACCGT119850.012.6032186
TAGAGCG139700.012.4785025
TATACTG241650.012.0887425
ACTGAGC216850.012.025248